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anti prrt1  (NeuroMab)


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    Structured Review

    NeuroMab anti prrt1
    Anti Prrt1, supplied by NeuroMab, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+prrt1/Anti-SynDIG4+Antibody/pmc08365426-38-11-12
    Average 90 stars, based on 2 article reviews
    anti prrt1 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Interaction and Subcellular Association of PRRT1/SynDIG4 With AMPA Receptors
    Article Snippet: The following primary antibodies were used: anti-PRRT1 (ProteinTech, 17261-1-AP, RRID:AB_2878371 ), anti-PRRT1 (NeuroMab, 75-409, RRID:AB_2531894 ), anti-GluA1 (Abcam, ab109450, RRID:AB_10860361 ), anti-GluA1 (NeuroMab, 75-327, RRID:AB_2315840 ), anti-MAP2 (EnCor, CPCA-MAP2, RRID:AB_2138173 ), anti-VGLUT1 (Synaptic Systems, 135 303, RRID:AB_887875 ), anti-EEA1 (Cell Signaling, C45B10, RRID:AB_2096811 ), anti-Rab7 (Cell Signaling, D95F2, RRID:AB_1904103 ), anti-Flag (Sigma, F3165, RRID:AB_259529 ), anti-HA (Thermo Fisher Scientific, 71-5500, RRID:AB_2533988 ), anti-HA (Biolegend, 16B12, RRID:AB_10064068 ), anti-GFP (Thermo Fisher Scientific, A-11122, RRID:AB_221569 ), and anti-β tubulin (Millipore, 05-661, RRID:AB_309885 ).



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    Synapse composition is unaltered in SynDIG1-deficient synapses. A , Representative immunoblots of biochemical fractions isolated from WT and SynDIG1 β-gal homozygous mutant P14 mouse brain tissue showing levels of GluA1, GluA2, GluN1, GluN2B, PSD-93, PSD-95, synaptophysin (Synapto), SynDIG1, <t>SynDIG4,</t> and PICK-1 present in the S1-, P2-, Syn-, and PSD-enriched fractions. Loading controls are provided by β-actin and β-tubulin immunoreactivity. B–D , Graphs depict the ratio of SynDIG1 β-gal homozygous mutant protein relative to WT levels of AMPA receptor subunits ( B ), NMDA receptor subunits ( C ), and PSD-93 and PSD-95 ( D ) in the PSD-enriched fractions. Data are the average of three independent biochemical fractionation experiments; each experiment used four to six mouse brains of each genotype. Error bars represent ±SEM.
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    Synapse composition is unaltered in SynDIG1-deficient synapses. A , Representative immunoblots of biochemical fractions isolated from WT and SynDIG1 β-gal homozygous mutant P14 mouse brain tissue showing levels of GluA1, GluA2, GluN1, GluN2B, PSD-93, PSD-95, synaptophysin (Synapto), SynDIG1, SynDIG4, and PICK-1 present in the S1-, P2-, Syn-, and PSD-enriched fractions. Loading controls are provided by β-actin and β-tubulin immunoreactivity. B–D , Graphs depict the ratio of SynDIG1 β-gal homozygous mutant protein relative to WT levels of AMPA receptor subunits ( B ), NMDA receptor subunits ( C ), and PSD-93 and PSD-95 ( D ) in the PSD-enriched fractions. Data are the average of three independent biochemical fractionation experiments; each experiment used four to six mouse brains of each genotype. Error bars represent ±SEM.

    Journal: eNeuro

    Article Title: Loss of SynDIG1 Reduces Excitatory Synapse Maturation But Not Formation In Vivo

    doi: 10.1523/ENEURO.0130-16.2016

    Figure Lengend Snippet: Synapse composition is unaltered in SynDIG1-deficient synapses. A , Representative immunoblots of biochemical fractions isolated from WT and SynDIG1 β-gal homozygous mutant P14 mouse brain tissue showing levels of GluA1, GluA2, GluN1, GluN2B, PSD-93, PSD-95, synaptophysin (Synapto), SynDIG1, SynDIG4, and PICK-1 present in the S1-, P2-, Syn-, and PSD-enriched fractions. Loading controls are provided by β-actin and β-tubulin immunoreactivity. B–D , Graphs depict the ratio of SynDIG1 β-gal homozygous mutant protein relative to WT levels of AMPA receptor subunits ( B ), NMDA receptor subunits ( C ), and PSD-93 and PSD-95 ( D ) in the PSD-enriched fractions. Data are the average of three independent biochemical fractionation experiments; each experiment used four to six mouse brains of each genotype. Error bars represent ±SEM.

    Article Snippet: Membranes were blotted with the following primary antibodies: mouse antibodies against PSD-95 [catalog #75-028, NeuroMab (RRID:AB_2292909)], synaptophysin [catalog #101011, Synaptic Systems (RRID:AB_887824)], SynDIG1 [catalog #75-251, NeuroMab (RRID:AB_10999753)], GluA2 [catalog #75-002, NeuroMab (RRID:AB_2232661)], PSD-93 [catalog #75-057, NeuroMab (RRID:AB_2277296)], β-tubulin [catalog #05-661, Millipore (RRID:AB_309885)], GluN1 [catalog #556308, BD Biosciences (RRID:AB_396353)], and Pick1 [catalog #73-040, NeuroMab (RRID:AB_10672986)]; rabbit antibodies against SynDIG4 (Anti-Prrt1, catalog #17261-1-AP, ProteinTech), β-actin [catalog #ab8224, Abcam (RRID:AB_449644)], GluA1 [catalog #AB1504, Millipore (RRID:AB_2113602)], and GluN2B (provided by J.W.H.

    Techniques: Western Blot, Isolation, Mutagenesis, Fractionation